| 翻訳と辞書 |
| Spin trapping ： ウィキペディア英語版 |
Spin trapping
Spin trapping is an analytical technique employed in chemistry and biology for the detection and identification of short-lived free radicals through the use of electron paramagnetic resonance (EPR) spectroscopy. EPR spectroscopy detects paramagnetism species such as the unpaired electrons of free radicals. However, when the half-life of radicals is too short to detect with EPR, compounds known as spin traps are used to react covalently with the radical products and form more stable adduct that will also have paramagnetic resonance spectra detectable by EPR spectroscopy. The use of radical-addition reactions to detect short-lived radicals was first proposed by E. G. Janzen in 1965.
==Spin traps==
The most commonly used spin traps are alpha-phenyl N-tertiary-butyl nitrone (PBN) and 5,5-dimethyl-pyrroline N-oxide (DMPO). More rarely, C-nitroso spin traps such as 3,5-Dibromo-4-nitrosobenzenesulfonic acid (DBNBS) can be used: often additional hyperfine information is derived, but at a cost of specificity (due to facile non-radical addition of many compounds to C-nitroso species, and subsequent oxidation of the resulting hydroxylamine).
5-Diisopropoxyphosphoryl-5-methyl-1-pyrroline-N-oxide (DIPPMPO) spin trapping has been used in measuring superoxide production in mitochondria.
抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』
■ウィキペディアで「Spin trapping」の詳細全文を読む
スポンサード リンク
| 翻訳と辞書 : 翻訳のためのインターネットリソース |
Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.
|
|